Recombinant Human Fc gamma RIIA/FCGR2A/CD32a Protein
The Recombinant Human Fc gamma RIIA/FCGR2A/CD32a Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Fc gamma RIIA/FCGR2A/CD32a function and its implications in health and disease.
This product (SKU: RPCB0542) is produced using E. coli and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 23.2 kDa with an observed molecular weight of 30-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Fc gamma RIIA/FCGR2A/CD32a Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Fc gamma RIIA/FCGR2A/CD32a Protein (RPCB0542), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Recombinant Human Fc gamma RIIA/FCGR2A/CD32a Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Human Fc gamma RIIA (H167) is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIA (H167), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 1.53 μM as determined in SPR assay (Biacore T200) (QC Test).
