The Recombinant Human FGF-2/bFGF Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating FGF-2/bFGF function and its implications in health and disease.
This product (SKU: RPCB0853) is produced using E. coli and features a No tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 16.41 kDa with an observed molecular weight of 17 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human FGF-2/bFGF Protein (RPCB0853), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of 20mM Tris，150 mM NaCl,pH7.5.Contact us for customized product form or formulation.
Bio-Activity:
Immobilized recombinant human FGF2 at 1 μg/mL (100 μL/well) can bind recombinant human FGFR4 with a linear range of 30-125 ng/mL.
Immobilized Human FGF2 at 0.5 μg/mL (100 μL/well) can bind Human GPC3 with a linear range of 7-20ng/mL.
Recombinant Human FGF-2 promotes the proliferation of Balb3T3 mouse embryonic fibroblasts cells. The ED 50 for this effect is 0.05-0.21 ng/mL, corresponding to a specific activity of 4.76×10 6 ~2.00×10 7 units/mg.
Human liver organoids were cultured with EGF, HGF(RPCB1637), FGF2(RPCB0853), FGF10(RPCB0828), NOG(RPCB0864), RSPO1(RPCB0176), WNT-3a(Cat. RP01618SLQ).
Human kidney organoids were cultured with EGF, FGF2(RPCB0853), FGF7(RPCB1793), FGF9(RPCB1059), FGF10(RPCB0828), IGF-(RPCB0759), NOG(RPCB0864), RSPO1(RPCB0176), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RPCB0891) was used to induce the establishment of cell polarity.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
This protein is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Recombinant Human FGF-2/bFGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized recombinant human FGF2 at 1 μg/mL (100 μL/well) can bind recombinant human FGFR4 with a linear range of 30-125 ng/mL.
Recombinant Human FGF-2 promotes the proliferation of Balb3T3 mouse embryonic fibroblasts cells. The ED50 for this effect is 0.05-0.21 ng/mL, corresponding to a specific activity of 4.76×106~2.00×107 units/mg.
Human liver organoids were cultured with EGF, HGF(RPCB1637), FGF2(RPCB0853), FGF10(RPCB0828), NOG(RPCB0864), RSPO1(RPCB0176), WNT-3a(Cat. RP01618SLQ).
