Description
Recombinant Human GAS-6 Protein
The Recombinant Human GAS-6 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating GAS-6 function and its implications in health and disease.
This product (SKU: RPCB2124) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 70.60 kDa with an observed molecular weight of 71-75 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human GAS-6 Protein |
| SKU: | RPCB2124 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | human |
| Synonyms: | GAS6, AXLLG, Growth arrest-specific protein 6, GAS-6, AXL receptor tyrosine kinase ligand |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 70.60 kDa |
| Observed MW: | 71-75 kDa |
| Gene ID: | 2621 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human GAS-6 Protein (RPCB2124), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 90 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
GAS-6 is a multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG). Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer. Human Gas6 interacts with both mouse and rat orthologs of these receptors. The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl. Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors. Gas6 induces a variety of responses, including prevention of apoptosis, cell proliferation, platelet-mediated thrombosis, retinal epithelial cell phagocytosis of outer rod segments, inhibition of VEGF-induced endothelial cell chemotaxis, and the differentiation and expansion of NK cell precursors. The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells. Several of these effects have been shown to require gamma -carboxylation of the Gla domain.
 | Recombinant Human GAS-6 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
