Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially identified as a growth factor that can aid in the cell proliferation of granulocyte-macrophage progenitors in culture. It is made by a variety of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts) in response to immune and inflammatory cytokines. GM-CSF is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF promotes endothelial cell migration and proliferation in human cells. Additionally, GM-CSF has been found to promote the proliferation of osteogenic sarcoma; carcinoma and adenocarcinoma cell lines in culture. Human GM-CSF/CSF2 Recombinant Protein (E.coli) is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human GM-CSF/CSF2 Recombinant Protein (E.coli) (RPES2386)
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 4-20 pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Glu144 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
14.6 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
