Immunoglobulin G (IgG) is the only monomeric immunoglobulin that can pass through the human placenta and is synthesized and released by plasma B cells. It constitutes 75% of serum immunoglobulins in humans, and it is a monomeric immunoglobulin involved in the secondary antibody response. IgG antibodies neutralize pathogens by agglutination and immobilization, complement activation, toxin neutralization, and antibody-dependent cell-mediated cytotoxicity. The IgG tetramer consists of two heavy chains (50 kDa) and two light chains (25 kDa), linked by disulfide bonds, with the two identical halves forming the Y-like form. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). The first immunoglobulin class in humans, IgG1 is most prevalent in blood and binds to Fc receptors (FcR ) on phagocytic cells with great affinity. For example, the Fc fragment of IgG is shown to mediate phagocytosis; cause inflammation, and selectively target Ig in specific tissues. Protein G or Protein A, which is on the surface of certain Staphylococcal and Streptococcal bacteria, specifically binds with the Fc region of IgGs; and it has several uses in biotechnology as a reagent for affinity purification. The recombinant immunoglobulin G Fc region is considered to be a potential anti-inflammatory therapy for human autoimmune diseases. Human IgG4-Fc Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human IgG4-Fc Recombinant Protein (RPES2134)
Product Code:
RPES2134
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Ig gamma-4 chain C region,IgG4 Fc,IGHG4
Accession:
P01861
Sequence:
Glu99-Gly326
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human IgG4-Fc is produced by our Mammalian expression system and the target gene encoding Glu99-Gly326 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
25.6 kDa
AP Mol Mass:
32 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
