Description
Recombinant Human IL-1R1/CD121a Protein
The Recombinant Human IL-1R1/CD121a Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating IL-1R1/CD121a function and its implications in health and disease.
This product (SKU: RPCB0530) is produced using HEK293 cells and features a No Tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 36.17 kDa with an observed molecular weight of 50-65 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human IL-1R1/CD121a Protein |
| SKU: | RPCB0530 |
| Size: | 100 μg |
| Reactivity: | Human |
| Synonyms: | IL-1R-1, IL-1RT1, IL-1R-alpha, CD121a, IL-1 RI, IL1R, IL1RI, IL-1RI, p80, D2S1473, IL1RA |
| Tag: | No Tag |
| Expression Host: | HEK293 cells |
| Calculated MW: | 36.17 kDa |
| Observed MW: | 50-65 kDa |
| Gene ID: | 3554 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human IL-1R1/CD121a Protein (RPCB0530), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC. |
| Formulation: | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Interleukin 1 (IL-1) has long been known for its pleiotropic effects on inflammation that plays a complex, and sometimes contrasting, role in different stages of cancer development.IL-1R1 is the main receptor for both ligands and is expressed by various cell types, including innate and adaptive immune cell types, epithelial cells, endothelial cells, adipocytes, chondrocytes, fibroblasts, etc. IL-1 and IL-1R1 receptor interaction leads to a set of common signaling pathways, mainly the NF-kB and MAP kinase pathways, as a result of complex positive and negative regulations.
 | Recombinant Human IL-1R1/CD121a Protein was determined by Tris-Bis PAGE under reducing conditions. |
 | The purity of Human IL-1R1 is greater than 95% as determined by SEC-HPLC. |
