Integrin ?-5 belongs to the Integrin ? chain family and contains 7 FG-GAP repeats. Integrin ?-5 joins with Integrin-β1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection; the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts; endothelial cells; peripheral T cells and platelets. Integrin ?-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion; ITGA5 participates in cell-surface mediated signalling.
Product Name:
Human ITGA5 Recombinant Protein (RPES1682)
Product Code:
RPES1682
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Integrin Alpha-5, CD49 Antigen-Like Family Member E, Fibronectin Receptor Subunit Alpha, Integrin Alpha-F, VLA-5, CD49e, ITGA5, FNRA
Accession:
P08648
Sequence:
Phe42-Tyr995
Fusion tag:
C-6His
Activity:
Immobilized Human NovoNectin(Cat: PKSH032450) at 2µg/ml(100 ?l/well) can bind Human ITGA5-His. The ED50 of Human ITGA5-His is 18.9 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Integrin ?-5 is produced by our Mammalian expression system and the target gene encoding Phe42-Tyr995 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
105.1 kDa
AP Mol Mass:
130 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
