The low density lipoprotein receptor (LDLR) gene family is made up of cell surface receptors that are involved in ligand-mediated endocytosis. The low-density lipoprotein (LDL) is tightly bound to the cell membrane and incorporated into the cell, where it is broken down and cholesterol is released for HMG CoA reductase repression, the rate-limiting step in cholesterol synthesis. Familial hypercholesterolemia is an autosomal dominant illness caused by defects in the LDLR gene. Hypercholesterolemia is an example of an comdition linked to LDLR. Lipid homeostasis and folate metabolism are related pathways of LDLR. Human LDLR Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Recombinant Human Low-Density Lipoprotein Receptor is produced by our Mammalian expression system and the target gene encoding Ala22-Arg788 is expressed with a AVI, 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
90.1 kDa
AP Mol Mass:
137 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 50mM HEPES, 150mM NaCl, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
