Recombinant Human Leukemia inhibitory factor/LIF Protein
The Recombinant Human Leukemia inhibitory factor/LIF Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Leukemia inhibitory factor/LIF function and its implications in health and disease.
This product (SKU: RPCB0190) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 20.56 kDa with an observed molecular weight of 33-45 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Leukemia inhibitory factor/LIF Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Leukemia inhibitory factor/LIF Protein (RPCB0190), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED 50 for this effect is typically 0.1-0.3 ng/mL, corresponding to a specific activity of 3.33×10 6 -1×10 7 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Recombinant Human Leukemia inhibitory factor/LIF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human LIF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.3 ng/mL, corresponding to a specific activity of 3.33×106-1×107units/mg.
