Description
Recombinant Human MMP-13 Protein
The Recombinant Human MMP-13 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating MMP-13 function and its implications in health and disease.
This product (SKU: RPCB0373) is produced using advanced expression systems and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 52.52 kDa with an observed molecular weight of 58 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human MMP-13 Protein |
| SKU: | RPCB0373 |
| Size: | 50 μg |
| Reactivity: | Human |
| Synonyms: | CLG3, MANDP1, MDST, MMP-13, MMP13, MANDP1, MDST, MMP-13 |
| Tag: | C-His |
| Calculated MW: | 52.52 kDa |
| Observed MW: | 58 kDa |
| Gene ID: | 4322 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human MMP-13 Protein (RPCB0373), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured in a cell migration assay using A549 cells. 10 ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration.2.Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >2900 pmol/min/μg. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is proteolytically processed to generate the mature protease. MMP-13 is distinguished from the other human collagenases,it cleaves type II collagen more efficiently than types I and III. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis.
 | Recombinant Human MMP-13 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Recombinant Human MMP-13 induces cell migration of the A549 cells. 10 ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration. |
