BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.
Product Name:
Rhesus/Canine BMP-2 Recombinant Protein (RPES1004)
Product Code:
RPES1004
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
BDA2,BDA2A,BMP-2,BMP2A,BMP2
Accession:
NP_001191.1
Sequence:
Gln 283-Arg 396
Fusion tag:
C-Fc
Activity:
1.Measured by its ability to bind recombinant human Nog-Fc in a functional ELISA.2. Measured by its ability to bind recombinant human ALK3-Fch in a functional ELISA.3. Measured by its ability to bind recombinant mouse ALK3-Fch in a functional ELISA.4. Measured by its ability to bind recombinant human BMPR-II-Fc in a functional ELISA.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of human BMP2 (NP_001191.1) (Gln 283-Arg 396) was fused with the Fc region of human IgG1 at the C-terminus. The mature form sequences of human, mouse, rat, rhesus and canine BMP2 are identical.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
39.5 kDa
Formulation:
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.51. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
