Neurocalcin-delta (NCALD) is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family. It expressed in mammalian brains. NCALD contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein possesses a Ca2+ /myristoyl switch. It is cytosolic at resting calcium levels. However, elevated intracellular calcium levels induce a conformational change which exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. NCALD protein is thought to be a regulator of G protein-coupled receptor signal transduction.
Product Name:
Neurocalcin-Delta/NCALD Recombinant Protein (RPES0259)
Product Code:
RPES0259
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Neurocalcin-Delta, NCALD
Accession:
P61601
Sequence:
Met 1-Phe193
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Neurocalcin-delta is produced by our E.coli expression system and the target gene encoding Met1-Phe193 is expressed with a 6His tag at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
24.4 kDa
AP Mol Mass:
20 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM DTT, 40% Glycerol, pH 8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
