Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein
The Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Neurotrophin-3/NGF-2/NT-3 function and its implications in health and disease.
This product (SKU: RPCB0520) is produced using E. coli. The protein exhibits a calculated molecular weight of 13.6 kDa with an observed molecular weight of 14 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein (RPCB0520), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 5.2.
Bio-Activity:
Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human TrkB (RPCB0492) at 2μg/mL (100 μL/well) can bind Recombinant Human Neurotrophin-3/NGF-2/NT-3 with a linear range of 1.95-35.5 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β -NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptideand a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequencesof mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survivalof specific neuronal subpopulations in both the central as well as the peripheral nervous systems.
Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human TrkB (RPCB0492) at 2μg/mL (100 μL/well) can bind Recombinant Human Neurotrophin-3/NGF-2/NT-3 with a linear range of 1.95-35.5 ng/mL.
