Neurturin is a GDNF-family ligand, which includes glial cell-derived neurotrophic factor (GDNF), neurturin, persephin, and artemin. Neurturin is found in both neurons and nonneuronal tissues. Neurturin is formed as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain, similarly to other TGF-beta family proteins. The carboxy terminal domain of neurturin contains the essential seven conserved cysteine residues for the formation of the cysteine-knot and single interchain disulfide bond. Biologically active human neurturin is a disulfide-linked homodimer of the carboxy-terminal 102 amino acid residues. Bioactivities of all GDNF family ligands are mediated by a distinct multicoelement receptor complex comprising a high-affinity ligand binding component (GFR alpha-1-GFR alpha-4) and a common signaling component (cRET receptor tyrosine kinase), which is distinct from other members of the TGF-beta Each member of the GDNF family of ligands binds to a unique protein. Neurturin binds to GFR alpha-2 with higher affinity than it does to GFR alpha-1. It may be involved in the development and maintenance of the central and peripheral nervous systems as well as non-neuronal systems. Human Neurturin/NRTN Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Neurturin/NRTN Recombinant Protein (RPES0395)
Product Code:
RPES0395
Size:
20 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Neurturin, NRTN
Accession:
Q99748
Sequence:
Ala96-Val197
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Neurturin is produced by our E.coli expression system and the target gene encoding Ala96-Val197 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
11.8 kDa
AP Mol Mass:
15 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 10mM sodium citrate, pH4.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
