Neutrophil cytosol factor 1( NCF1) is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is characterized as a multicomponent enzyme which is activated to produce superoxide anion. NCF2, NCF1, and a membrane bound cytochrome b558 are required for the activation of the latent NADPH oxidase. The human NCF1 gene encodes a 390 amino acids protein without a signal peptide. The NCF1 gene interacts with other subunits of nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) and plays an important role in innate immunity, producing reactive oxygen species and reducing the severity and duration of parasitic infection and autoimmune disease. NCF1 also has a role in T cell activation.
Product Name:
NCF1 Recombinant Protein (RPES0415)
Product Code:
RPES0415
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
NCF-1,47 kDa autosomal chronic granulomatous disease protein/47 kDa neutrophil oxidase factor,NCF-47K,Neutrophil NADPH oxidase factor 1,Nox organizer 2,Nox-organizing protein 2/SH3 and PX domain-containing protein 1A,p47-phox
Accession:
P14598
Sequence:
Met 1-Val390
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Neutrophil cytosol factor 1 is produced by our E.coli expression system and the target gene encoding Met1-Val390 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
45.6 kDa
AP Mol Mass:
45-50 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris 100mM NaCl,1mM DTT, pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
