Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family, which includes a number of other members. Platelet-derived growth factor is a mitogen for mesenchymal cell lines. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Meningioma is linked to a variety of mutations in this gene. PDGFB binds to its receptor, which stimulates a variety of cellular responses. PDGFB is also released by platelets during injury, and it serves an essential function in encouraging adjacent cells to proliferate and heal the wound. Human PDGF-BB Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 15-60ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Platelet-Derived Growth Factor BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
12.4 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 4mM HCl.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
