Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
Measured by its binding ability in a functional ELISA.2.Immobilized human PDGFB at 10µg/mL (100?L/well) can bind human PDGFR?-Fch, the EC50 of human PDGFR?-Fch is 0.05-0.2 µg/mL.
Endotoxin:
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Protein Construction:
A DNA sequence encoding the human PDGFB (NP_148937.1) (Ser82-Thr190) was expressed with a polyhistidine tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
14.3 kDa
Formulation:
Lyophilized from sterile PBS, 25 % Glycerol, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
