The Recombinant Human Plasma Kallikrein/KLKB1 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Plasma Kallikrein/KLKB1 function and its implications in health and disease.
This product (SKU: RPCB1881) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 70.3 kDa with an observed molecular weight of 72-80 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human Plasma Kallikrein/KLKB1 Protein (RPCB1881), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of 20mM NaAc,150mM NaCl,pH5.0.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in 20mM NaAc,150mM NaCl,pH5.0.. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.
Recombinant Human Plasma Kallikrein/KLKB1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Human Plasma Kallikrein/KLKB1 Protein (Cat.RP02872LQ) was greater than 95% as determined by SEC-HPLC.
