RRM2 is one of two non-identical ribonucleotide reductase subunits. RRM2 is responsible formation of deoxyribonucleotides from ribonucleotides. The encoded protein (M2) is generated via a cell-cycle-dependent process. RRM2 transcription can start from two different promoters, resulting in two isoforms with different N-termini lengths. It's also been observed on chromosomes 1 and X that RRM2 pseudogenes have been identified. Choriocarcinoma and Diamond-Blackfan Anemia 20 are two diseases related to RRM2. Pyrimidine metabolism and E2F-mediated DNA replication regulation are two related pathways to RRM2. Human Protamine-2/PRM2 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Protamine-2/PRM2 Recombinant Protein (RPES3025)
Product Code:
RPES3025
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Ribonucleoside-Diphosphate Reductase Subunit M2, Ribonucleotide Reductase Small Chain, Ribonucleotide Reductase Small Subunit, RRM2, RR2
Accession:
P31350
Sequence:
Met 1-Phe389
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Ribonucleotide Reductase Small Chain is produced by our Mammalian expression system and the target gene encoding Met1-Phe389 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
45.9 kDa
AP Mol Mass:
47 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
