The Recombinant Human R-spondin-2/RSPO2 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating R-spondin-2/RSPO2 function and its implications in health and disease.
This product (SKU: RPCB2027) is produced using advanced expression systems and features a C-hFC tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 47.66 kDa with an observed molecular weight of 55-60 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human R-spondin-2/RSPO2 Protein (RPCB2027), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its ability to induce Topflash reporter activity in 293 human embryonic kidney cells. The typical ED 50 is 86 ng/mL in the presence of 3.24 ng/mL Recombinant Human Wnt3a Surrogate.
Reconstitution:
Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
R-spondins are secretory proteins localized in the endoplasmic reticulum and Golgi bodies and are processed through the secretory pathway. The RSPO family comprises four proteins, i.e., R-spondin 1-4, which are important secreted regulators of the Wnt/å°¾-catenin and Wnt/PCP signaling pathway and govern various functions and are involved in vital cellular processes such as stem cell renewal, morphogenesis and, tumorigenesis. Among the R-spondin family, R-spondin-2, is a key protein that regulates ligand-dependent å°¾-catenin signaling. And it has been reported to play a pivotal role in myogenesis, osteoblast differentiation, and limb specification during embryonic development.
Recombinant Human R-spondin-2/RSPO2 Protein was determined by SDS-PAGE under reducing (R)
