S100A11 is a member of the S100 family of calcium binding proteins. Human S100A11 is a protein that has two EF hand motifs and 82 percent amino acid sequence similarity with mouse and rat S100A11. It forms covalent heterodimers and tetramers via transglutamination, as well as disulfide-linked tetramers. Keratinocytes produce S100A11, which can be crosslinked into the cornified envelope of the skin. Dimerization enhances its ability to signal through RAGE on keratinocytes, induce the production of EGF family proteins, and induce cell proliferation. S100A11 activates RAGE on chondrocytes, causing hypertrophy and catabolism of the cartilage matrix via dimerization. In the cytosol, S100A11 is phosphorylated and translocates to the nucleus in response to DNA damage, RELM alpha exposure, or heightened extracellular calcium concentrations. Calcium encourages S100A11 binding to S100B and Annexins A1, A2, and A6. Annexin A2 complexes are recruited to sites of plasma membrane damage, where they help cancer cells migrate. S100A11 is upregulated in various cancers and supports tumor cell proliferation, invasion, and migration. S100A11 is also made in the ovary, where it inhibits oocyte fertilization through cumulus cells. Human S100A11 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human S100A11 Recombinant Protein (RPES0227)
Product Code:
RPES0227
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Protein S100-A11, Calgizzarin, Metastatic lymph node gene 70 protein, MLN 70, Protein S100-C, S100 calcium-binding protein A11, S100A11, MLN70, S100C
Accession:
P31949
Sequence:
Met1-Thr105
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human S100A11 is produced by our E.coli expression system and the target gene encoding Met1-Thr105 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
11.7 kDa
AP Mol Mass:
12 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS,1mM DTT,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
