The Recombinant Human Serpin H1/HSP47 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Serpin H1/HSP47 function and its implications in health and disease.
This product (SKU: RPCB0647) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 45.5 kDa with an observed molecular weight of 55-60 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human Serpin H1/HSP47 Protein (RPCB0647), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.Contact us for customized product form or formulation.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Serpin H1 is a serine proteinase inhibitors Which belongs to the serpin family. Serpin H1 is induced by heatshock. Serpin H1 localizes to the endoplasmic reticulum lumen and binds specifically to collagen. Thus it isthought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to thisprotein have been found in patients with rheumatoid arthritis. Serpin H1 may be a marker for cancer andnucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm prematurerupture of membranes.
Recombinant Human Serpin H1/HSP47 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
