Human serum albumin (HSA), the most abundant protein in human blood plasma, is essential for maintaining osmotic pressure. It is produced in the liver, consists of a single polypeptide chain, with 585 amino acids with 17 tyrosil residues and one tryptophan located in position 214. HSA is organized in three domains, I, II and III, each consisting of two subdomains, A and B. In the physiological states, HSA occurs in two forms – the non-modified HSA with a free thiol group of Cys-34, and the modified (oxidized) form (oHSA), also called human mercaptoalbumin (HMA) and human nonmercaptoalbumin (HNA), respectively. HMA and HNA are in equilibrium, depending on the redox state of Cys-34, and their ratio also varies depending on age and the diseased state. HSA functions primarily as a carrier protein for drugs, steroids, fatty acids, and thyroid hormones, and plays a role in stabilizing extracellular fluid volume.
Product Name:
Human Serum Albumin/HSA Recombinant Protein (RPES4846)
Product Code:
RPES4846
Size:
10µg
Species:
Human
Expressed Host:
P.Pichia
Synonyms:
Serum Albumin, HAS, ALB
Accession:
P02768
Sequence:
Asp25-Leu609
Fusion tag:
C-8His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Serum Albumin is produced by our Yeast expression system and the target gene encoding Asp25-Leu609 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
66.5 kDa
AP Mol Mass:
55-80 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
