Recombinant Human TGF-beta receptor type-2/TGFR-2 Protein
The Recombinant Human TGF-beta receptor type-2/TGFR-2 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating TGF-beta receptor type-2/TGFR-2 function and its implications in health and disease.
This product (SKU: RPCB0334) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 42.30 kDa with an observed molecular weight of 55-75 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human TGF-beta receptor type-2/TGFR-2 Protein
High quality, high purity and low endotoxin recombinant Recombinant Human TGF-beta receptor type-2/TGFR-2 Protein (RPCB0334), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 μg/mL (100 μL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.
Recombinant Human TGF-beta receptor type-2/TGFR-2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized recombinant human TGFB1 at 2 μg/mL (100 μL/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL.
