Description
Recombinant Human TNF-alpha Protein
The Recombinant Human TNF-alpha Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating TNF-alpha function and its implications in health and disease.
This product (SKU: RPCB0806) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 18.19 kDa with an observed molecular weight of 18 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human TNF-alpha Protein |
| SKU: | RPCB0806 |
| Size: | 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | TNF, DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor, TNF-α, DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F, TNF alpha |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 18.19 kDa |
| Observed MW: | 18 kDa |
| Gene ID: | 7124 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human TNF-alpha Protein (RPCB0806), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Recombinant Human TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED 50 for this effect is 0.90-3.60 ng/mL, corresponding to a specific activity of 2.78×10 5 ~1.11×10 6 units/mg. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Tumor necrosis factor alpha (TNF-alpha), also kNown as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. KNockout studies in mice also suggested the neuroprotective function of this cytokine.
 | Recombinant Human TNF-alpha Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Recombinant Human TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.90-3.60 ng/mL, corresponding to a specific activity of 2.78×105~1.11×106 units/mg. |
