Tumor Necrosis Factor beta (TNF-beta) is a secreted protein belonging to the tumor necrosis factor family. TNF-beta binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM in homotrimeric form, while it binds to TNFRSF3/LTBR in heterotrimeric form with LTB. TNF-beta forms heterotrimers with lymphotoxin-beta, which anchors TNF-beta to the cell surface. TNF-beta mediates the inflammatory, immunostimulatory, and antiviral response, involves in the formation of second lymphoid organs during development, has a role in apoptosis. TNF-beta is produced by lymphocytes and cytotoxic for a variety of tumor cells in vitro and in vivo. Human TNF beta/TNFB Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human TNF beta/TNFB Recombinant Protein (RPES1684)
Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 20-80 pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Tumor Necrosis Factor beta is produced by our E.coli expression system and the target gene encoding Leu35-Leu205 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
18.8 kDa
AP Mol Mass:
15 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
