Tumor Necrosis Factor Receptor Superfamily Member 10C (TNFRSF10C) is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL with high affinity. TNFRSF10C has the TRAIL-binding extracellular cysteine-rich domains; lacks the intracellular signaling domain. As a result; binding of TRAIL to TRAIL R3 doesnÂ’t transduce an apoptosis signal. The expression of TRAIL R3 gene has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis.
Product Name:
Human TRAIL R3/TNFRSF10C Recombinant Protein (RPES1041)
Product Code:
RPES1041
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Tumor Necrosis Factor Receptor Superfamily Member 10C, Antagonist Decoy Receptor for TRAIL/Apo-2L, Decoy TRAIL Receptor Without Death Domain, Decoy Receptor 1, DcR1, Lymphocyte Inhibitor of TRAIL, TNF-Related Apoptosis-Inducing Ligand Receptor 3, TRAIL Receptor 3, TRAIL-R3, TRAIL Receptor Without an Intracellular Domain, CD263, TNFRSF10C, DCR1, LIT, TRAILR3, TRID
Accession:
O14798
Sequence:
Ala26-Ala221
Fusion tag:
C-6His
Activity:
Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L?929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 0.56 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human TNF-Related Apoptosis-Inducing Ligand Receptor 3 is produced by our Mammalian expression system and the target gene encoding Ala26-Ala221 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
21.8 kDa
AP Mol Mass:
40 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
