Description
Recombinant Human Trk-B/NTRK2 Protein
The Recombinant Human Trk-B/NTRK2 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Trk-B/NTRK2 function and its implications in health and disease.
This product (SKU: RPCB0492) is produced using advanced expression systems and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 45.05 kDa with an observed molecular weight of 55-70 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human Trk-B/NTRK2 Protein |
| SKU: | RPCB0492 |
| Size: | 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | NTRK2, GP145-TrkB, TRKB, trk-B, BDNF/NT-3 growth factors receptor, GP145-TrkB, TRKB, trk-B, TrkB, OBHD |
| Tag: | C-His |
| Calculated MW: | 45.05 kDa |
| Observed MW: | 55-70 kDa |
| Gene ID: | 4915 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Trk-B/NTRK2 Protein (RPCB0492), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant HumanTrkB, His Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human BDNF with a linear range of 1.95-258 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family and contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain.TrkB/NTRK2 tyrosine kinase is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways.TrkB/NTRK has high affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. As a membrane-bound receptor, TrkB/NTRK binds upon neurotrophin, phosphorylates itself and members of the MAPK pathway. NTRK2 / TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.
 | Recombinant Human Trk-B/NTRK2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Recombinant Human TrkB (RPCB0492) at 2μg/mL (100 μL/well) can bind Recombinant Human BDNF(RPCB1511) with a linear range of 1.95-258ng/mL. |
