NKG2D Ligand 2 (N2DL2) is a member of a family of cell-surface proteins. N2DL2 function as ligands for human cytomegalovirus glycoprotein UL16. N2DL2 is anchored to the membrane via a GPI-linkage. N2DL2 is bind to human NKG2D; an activating receptor expressed on NK cells; NKT cells; T cells. Engagement of NKG2D results in the activation of cytolytic activity and cytokine production by these effects cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance.
Product Name:
Human ULBP2/N2DL-2 Recombinant Protein (RPES0626)
Product Code:
RPES0626
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
NKG2D Ligand 2, N2DL-2, NKG2DL2, ALCAN-Alpha, Retinoic Acid Early Transcript 1H, UL16-Binding Protein 2, ULBP2, N2DL2, RAET1H
Accession:
Q9BZM5
Sequence:
Gly26-Ser217
Fusion tag:
C-Fc
Activity:
Immobilized Human NKG2DL2-Fc at 8µg/ml(100 ?l/well) can bind Human NKG2D-His(Cat: PKSH033409). The ED50 of Human NKG2DL2-Fc is 10.32 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human UL16 Binding Protein-2/NKG2D ligand 2 is produced by our Mammalian expression system and the target gene encoding Gly26-Ser217 is expressed with a Fc tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
51.4 kDa
AP Mol Mass:
55 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
