vesicle-associated membrane protein-associated protein B/C (VAPB) is presents in mitochondria-associated membranes that are endoplasmic reticulum membrane regions closely apposed to the outer mitochondrial membrane. It can form homodimer; and heterodimer with VAPA. It also interacts with VAMP1; VAMP2; HCV NS5A; NS5B; ZFYVE27 and RMDN3. VAPB participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. It is involved in cellular calcium homeostasis regulation.
Product Name:
Human VAPB Recombinant Protein (RPES3595)
Product Code:
RPES3595
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Vesicle-associated membrane protein-associated protein B/C,VAMP-B/VAMP-C,VAMP-associated protein B/C,VAP-B/VAP-C
Accession:
O95292-1
Sequence:
Ala2-Pro132
Fusion tag:
C-6His
Activity:
Immobilized Human EphB2-Fc(Cat: PKSH032012) at 1µg/ml(100 ?l/well) can bind Human VAMPB-His. The ED50 of Human VAMPB-His is 5.179ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Vesicle-associated membrane protein-associated protein B is produced by our E.coli expression system and the target gene encoding Ala2-Pro132 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
16.0 kDa
AP Mol Mass:
16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
