The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
Product Name:
Mouse CA2 Recombinant Protein (RPES0292)
Product Code:
RPES0292
Size:
50µg
Species:
Mouse
Expressed Host:
E.coli
Synonyms:
AI131712,Ca2,CAII,Car-2,Ltw-5,Lvtw-5
Accession:
AAH55291.1
Sequence:
Met 1-Lys 260
Fusion tag:
C-His
Activity:
Measured by its esterase activity. The specific activity is >100 pmoles/min/µg.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mouse CA2 (AAH55291.1) (Met 1-Lys 260) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 96 % as determined by SDS-PAGE
Mol Mass:
30.4 kDa
AP Mol Mass:
30-33 kDa
Formulation:
Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
