Ectonucleoside triphosphate diphosphohydrolase-1(NTPDase-1)is an integral membrane protein with an extracellular active site. Recombinant mouse NTPDase-1wasexpressed as a protein lacking its N- andC-terminaltransmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-1was originally describedas CD39, a B lymphocyte cell surface marker. but it is also present on the surface of natural killer cells, T cells, and some endothelial cells. NTPDase1hydrolyzes the β-and? phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1plays arole in the regulation of purinergic signaling. NTPDase-1is involved in the processes of thromboregulation and vascular inflammation. The administration ofsoluble NTPDase-1may have therapeutic applications for the treatment of some vascular and transplantation-associateddiseases.
Recombinant Mouse ENTPD1 is produced by our Mammalian expression system and the target gene encoding Thr38-Ile478 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
50.5 kDa
AP Mol Mass:
60-90 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris,500mM NaCl,10%Glycerol,pH7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
