Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.
Product Name:
Mouse EphB4/HTK Recombinant Protein (RPES1036)
Product Code:
RPES1036
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AI042935,Htk,MDK2,Myk1,Tyro11
Accession:
P54761-1
Sequence:
Met 1-Ala 539
Fusion tag:
C-Fc
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse EPHB2-His at 10 µg/mL (100 ?l/well) can bind mouse EPHB4-Fc , The EC50 of mouse EPHB4-Fc is 15 ng/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse EPHB4 (P54761-1) extracellular domain (Met 1-Ala 539) was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
84.7 kDa
AP Mol Mass:
110 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
