Description
Recombinant Mouse Fc gamma RI/FCGR1/CD64 Protein
The Recombinant Mouse Fc gamma RI/FCGR1/CD64 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate Fc gamma RI/FCGR1/CD64 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB0717) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 31.5 kDa with an observed molecular weight of 40-60 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse Fc gamma RI/FCGR1/CD64 Protein |
| SKU: | RPCB0717 |
| Size: | 10 μg |
| Reactivity: | Mouse |
| Synonyms: | High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, CD64, Fcgr1 |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 31.5 kDa |
| Observed MW: | 40-60 kDa |
| Gene ID: | 14129 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse Fc gamma RI/FCGR1/CD64 Protein (RPCB0717), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation. |
| Reconstitution: | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
CD64, also known as Fc-gamma receptor 1 (Fc γ RI), is a type of integral membrane glycoprotein that bindsmonomeric IgG-type antibodies with high affinity. After binding IgG, CD64 interacts with an accessory chainknown as the common γ chain (γ chain), which possesses an ITAM motif that is necessary for triggering cellularactivation. CD64 is composed of a signal peptide, three extracellular immunoglobulin domains of the C2-typeused to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 mediatesendocytosis, phagocytosis, antibody-dependent cellular cytotoxicity, cytokine release, and superoxideproduction. It is normally expressed on the surfaces of monocytes and macrophages.
 | Recombinant Mouse Fc gamma RI/FCGR1/CD64 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
