The Recombinant Mouse FGF-2/bFGF Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate FGF-2/bFGF biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB0896) is produced using E. coli and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 17.06 kDa with an observed molecular weight of 22 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse FGF-2/bFGF Protein (RPCB0896), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Active Recombinant Mouse FGF2 was added during DP cell culture. The immunofluorescence identification showed that 1 ng/mL FGF2 could maintain the cellular characteristics of DP cells. The red part is the fluorescently labeled PDFGRA antibody, and the blue-cyan part is the DAPI-stained cells.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.
Recombinant Mouse FGF-2/bFGF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED50 for this effect is 0.065-0.262 ng/mL, corresponding to a specific activity of 3.82×106~1.54×107 units/mg.
