The Recombinant Mouse IL-15 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate IL-15 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1732) is produced using E. coli and features a C-6His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 14.09 kDa with an observed molecular weight of 14 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse IL-15 Protein (RPCB1732), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured in a cell proliferation assay using HT-2 mouse T cells. The ED 50 for this effect is 15-58 ng/mL, corresponding to a specific activity of 1.72×10 4 -6.67×10 4 units/mg.2.Measured in a cell proliferation assay using CTLL‑2 mouse T cells. The ED50 for this effect is 8.1‑32.4ng/mL,corresponding to a specific activity of 3.09×104~1.23×105units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Interleukin 15 (IL-15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules.
Recombinant Mouse IL-15 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Mouse IL-15 stimulates cell proliferation of the HT-2 mouse T cells. The ED50 for this effect is 15-58 ng/mL, corresponding to a specific activity of 1.72×104-6.67×104 units/mg.
