The Recombinant Mouse LAG-3/CD223 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate LAG-3/CD223 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1215) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 46.18 kDa with an observed molecular weight of 55-65 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse LAG-3/CD223 Protein (RPCB1215), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA.Immobilized Mouse LAG3 (RPCB1215) at 1 μg/mL (100 μL/well) can bind LAG3 Rabbit pAb with a linear range of 0.06-1.2ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
LAG3 (Lymphocyte Activating 3), also known as CD223 and Lymphocyte activation gene 3, belongs to the immunoglobulin (Ig) superfamily, is a protein-coding gene. 2 alternatively spliced human isoforms have been reported. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 contains 4 extracellular Ig-like domains and has a negative regulatory function in T cells. It also acts as a new marker of T cell-induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling, leading to increased antigen-specific T-cell responses in vivo. Diseases associated with LAG3 include Smoldering Myeloma and Kyphoscoliotic Heart Disease.
Recombinant Mouse LAG-3/CD223 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Mouse LAG3 (RPCB1215) at 1 μg/mL (100 μL/well) can bind LAG3 Rabbit pAb with a linear range of 0.06-1.2ng/mL.
