SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
Product Name:
Mouse Smad5 Recombinant Protein (RPES4961)
Product Code:
RPES4961
Size:
100 µg
Species:
Mouse
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
1110051M15Rik,AI451355,Dwf-C,Madh5,MusMLP
Accession:
P97454
Sequence:
Thr2-Ser465
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse SMAD5 (P97454) (Thr2-Ser465) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
52.2 kDa
AP Mol Mass:
57 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 500mM Nacl,10% glycerol, pH8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
