Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. TNF-alpha Protein & AntibodyImmune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
Product Name:
Mouse TNF-alpha/TNFA Recombinant Protein (RPES4097)
Measured in a cytotoxicity assay using L929µmouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the soluble form of mouse TNF-? (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the C-terminus.
Purity:
> 98 % as determined by SDS-PAGE
Mol Mass:
17 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from sterile 50mM Tris 0.2M NaCl, pH 7.2
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
