Description
Recombinant Mouse TREM2 Protein
The Recombinant Mouse TREM2 Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate TREM2 biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1621) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 17.65 kDa with an observed molecular weight of 25-40 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Mouse TREM2 Protein |
| SKU: | RPCB1621 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Mouse |
| Synonyms: | TREM-2, Trem2a, Trem2b, Trem2c, TREM2 |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 17.65 kDa |
| Observed MW: | 25-40 kDa |
| Gene ID: | 83433 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse TREM2 Protein (RPCB1621), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | Please contact us for more information. |
| Purity: | ≥ 90 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Mouse TREM-2 Protein at 1 μg/mL (100 μL/well) can bind TREM-2 Rabbit pAb with a linear range of 0.06-11.7 ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
Triggering Receptor Expressed on Myeloid cells 2 (TREM2)is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 and inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes.It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria
 | Recombinant Mouse TREM2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized Mouse TREM-2 Protein at 1 μg/mL (100 μL/well) can bind TREM-2 Rabbit pAb with a linear range of 0.06-11.7 ng/mL |
