TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy
Product Name:
Mouse TrkB/NTRK2 Recombinant Protein (RPES3677)
Product Code:
RPES3677
Size:
50 µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
GP145-TrkB/GP95-TrkB,Tkrb,trk-B,trkB
Accession:
P15209-2
Sequence:
Met 1-His 429
Fusion tag:
C-His
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized recombinant Mouse/Human BDNF Protein (Native) at 10 µg/ml (100 ?l/well) can bind biotinylated mouse TrkB-His with a linear range of 10-80 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse NTRK2 (P15209-2) (Met 1-His 429) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 98 % as determined by SDS-PAGE
Mol Mass:
46 kDa
AP Mol Mass:
75-85 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
