The Recombinant Rat ALK-1/ACVRL1 Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB2030) is produced using advanced expression systems and features a C-hFc tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 36.97 kDa with an observed molecular weight of 45-60 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Rat ALK-1/ACVRL1 Protein
SKU:
RPCB2030
Size:
10 μg , 20 μg , 50 μg , 100 μg
Reactivity:
Rat
Synonyms:
Acvrl1, Acvrlk1, Serine/threonine-protein kinase receptor R3, SKR3, 2.7.11.30, TGF-B superfamily receptor type I, TSR-I
High quality, high purity and low endotoxin recombinant Recombinant Rat ALK-1/ACVRL1 Protein (RPCB2030), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Rat ALK-1(Catalog #RPCB2030) at 4 μg/mL (100 μL/well) can bind Human ACVR2B (Catalog #RPCB0375) with a linear range of 20-989.42 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ALK-1/ACVRL1,Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
Recombinant Rat ALK-1/ACVRL1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Rat ALK-1(Catalog #RPCB2030) at 4 μg/mL (100 μL/well) can bind Human ACVR2B (Catalog #RPCB0375) with a linear range of 20-989.42 ng/mL.
