The Recombinant Rat MMP-9 Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB0893) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 75.55 kDa with an observed molecular weight of 90-100 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Rat MMP-9 Protein
SKU:
RPCB0893
Size:
50 μg , 100 μg
Reactivity:
Rat
Synonyms:
92 kDa gelatinase, 92 kDa type IV collagenase, GELB, MMP9, MMP-9, MMP9
High quality, high purity and low endotoxin recombinant Recombinant Rat MMP-9 Protein (RPCB0893), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of 50mM Tris,10mM CaCl2,150mM NaCl, pH 7.5.Contact us for customized product form or formulation.
Bio-Activity:
Measured in a cell migration assay using Hela cells. 1 ng/mL of Recombinant Rat MMP-9 can effectively induce Hela cells migration.
Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >1600 pmoles/min/μg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Rat MMP-9 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Recombinant Rat MMP9 induces cell migration of the Hela cells. 1 ng/mL of Recombinant Rat MMP-9 can effectively induce Hela cells migration.
