Recombinant SARS-CoV-2 Nucleocapsid (G335A) Protein
The Recombinant SARS-CoV-2 Nucleocapsid (G335A) Protein represents a critical molecular component of the SARS-CoV-2 virus, playing an essential role in viral entry and host cell infection mechanisms. This protein is extensively utilized in COVID-19 research, vaccine development, therapeutic antibody screening, and diagnostic assay development, serving as a fundamental tool for understanding viral pathogenesis and developing countermeasures against coronavirus infections.
This product (SKU: RPCB1358) is produced using Baculovirus-Infected Sf9 Cells and features a N-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 46.35 kDa with an observed molecular weight of 55 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant SARS-CoV-2 Nucleocapsid (G335A) Protein
High quality, high purity and low endotoxin recombinant Recombinant SARS-CoV-2 Nucleocapsid(G335A) Protein (RP01281LQ), tested reactivity in Baculovirus-Infected Sf9 Cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Supplied as a 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 0.1mM EDTA, 10% glycerol, pH8.0.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Nucleocapsid Protein at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 N Protein Rabbit mAb with a linear range of 0.032-0.79 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC 50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 46.90 ng/mL.
Storage:
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Recombinant SARS-CoV-2 Nucleocapsid(G335A) Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Recombinant SARS-CoV-2 Nucleocapsid Protein at 2μg/mL (100 μL/well) can bind SARS-CoV-2 N Protein Rabbit mAb with a linear range of 0.032-0.79 ng/mL.
