Recombinant SARS-CoV-2 Spike S1+S2 ECD (S-ECD) (D614G) Protein
The Recombinant SARS-CoV-2 Spike S1+S2 ECD (S-ECD) (D614G) Protein represents a critical molecular component of the SARS-CoV-2 virus, playing an essential role in viral entry and host cell infection mechanisms. This protein is extensively utilized in COVID-19 research, vaccine development, therapeutic antibody screening, and diagnostic assay development, serving as a fundamental tool for understanding viral pathogenesis and developing countermeasures against coronavirus infections.
This product (SKU: RPCB1359) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 133.52 kDa with an observed molecular weight of 180-200 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant SARS-CoV-2 Spike S1+S2 ECD (S-ECD) (D614G) Protein
High quality, high purity and low endotoxin recombinant Recombinant SARS-CoV-2 Spike S1+S2 ECD(S-ECD)(D614G) Protein (RP01291LQ), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant SARS-CoV-2 Spike S1+S2 ECD at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-2.58 ng/mL.
Storage:
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Recombinant SARS-CoV-2 Spike S1+S2 ECD(S-ECD)(D614G) Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized recombinant SARS-CoV-2 Spike S1+S2 ECD at 2μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-2.58 ng/mL.
