Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
Product Name:
S. hygroscopicus Bar Recombinant Protein (RPES4076)
Product Code:
RPES4076
Size:
10µg
Species:
Streptomyces hygroscopicus
Expressed Host:
E.coli
Synonyms:
Phosphinothricin N-acetyltransferase, PPT N-acetyltransferase, Phosphinothricin-resistance protein, bar
Accession:
P16426
Sequence:
Met1-Ile183
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Streptomyces hygroscopicus Bar is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
20.6 kDa
AP Mol Mass:
18-20 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
