The REST/NRSF Monoclonal Antibody (CAB2415) is a high-quality antibody developed for reliable detection and analysis of target proteins. This protein plays a critical role in gene regulation, specifically in neural development and function.Raised in rabbits, this monoclonal antibody is highly specific and reactive with human samples, making it a valuable tool for studies in neuroscience, neurology, and related fields. It has been validated for use in Western blot applications, allowing for the detection and analysis of REST/NRSF protein levels in various cell types and tissues.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
REST/NRSF Monoclonal Antibody
SKU:
CAB2415
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0755
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
KGEP HGLE NMEL RSLE LSVV EPQP VFEA SGAP DIYS SNKD LPPE TPGA EDKG KSSK TKPF RCKP CQYE AESE EQFV HHIR VHSA KKFF VEES AEKQ AKAR E
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
WT6, XBR, HGF5, NRSF, DFNA27, GINGF5, REST/NRSF
Positive Sample:
HeLa, U-87MG, Mouse brain, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
122kDa
Observed MW:
160kDa
This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
5978
RRID
AB_2863005
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using REST/NRSF Rabbit mAb (CAB2415) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Rat brain, using REST/NRSF Rabbit mAb (CAB2415) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
