The RGL3 Polyclonal Antibody (AB10001) is specially developed for researchers investigating RGL3, a signaling protein known for its involvement in cellular processes such as cell growth and differentiation. Produced in rabbits, this antibody demonstrates high reactivity with human samples and is validated for use in various applications including Western blotting.RGL3, also referred to as a regulator of G-protein signaling 3, plays a key role in the regulation of G-protein coupled receptor signaling pathways, making it an important target for studies in cell biology and signal transduction.
The antibody's ability to specifically bind to RGL3 allows for accurate detection and analysis in different cell types, making it an ideal tool for research in areas like cancer biology and neurology.Understanding the function and regulation of RGL3 is essential for unraveling its role in various physiological and pathological conditions. With its versatility and reliability, the RGL3 Polyclonal Antibody is a valuable resource for researchers seeking to explore the complex mechanisms involving RGL3 and its impact on cellular signaling pathways.
Encodes a DELLA subfamily member that acts as a negative regulator of GA signaling and as a coactivator of ABI3 to promote seed storage protein biosynthesis during the seed maturation stage. Involved in hyperosmotic salinity response, jasmonic acid mediated signaling pathway, negative regulation of gibberellic acid mediated signaling pathway, negative regulation of seed germination, regulation of DNA-templated transcription, regulation of reactive oxygen species metabolic process, regulation of seed dormancy process, response to abscisic acid, response to ethylene, salicylic acid mediated signaling pathway.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using RGL3 Rabbit pAb (CAB22552) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
