The Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L) (CABS040) is a high-quality antibody developed for reliable detection and analysis of target proteins. This highly reactive antibody, produced in goats, is specifically tailored for use with rabbit immunoglobulins and is ideal for applications such as immunofluorescence staining and flow cytometry. By binding to rabbit IgG antibodies, this conjugated antibody allows for enhanced visualization and detection of target proteins in various experimental settings.The Rhodamine TRITC conjugation provides a bright and stable fluorescent signal, making it a valuable tool for research in fields such as cell biology, molecular biology, and immunology.
This antibody is validated for use in IHC-P, IF/ICC, FC applications and has demonstrated reactivity against Rabbit samples.
This information is considered to be commercially sensitive.
Tested Applications:
IHC-PIF/ICCFC
Recommended Dilution:
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
FC
1:50 - 1:200
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2772013
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol,pH7.3.
Immunofluorescence analysis of HeLa cells, using Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (CAB3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (CABS040) at 1:200 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells, using Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (CAB0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L) (CABS040) at 1:100 dilution. Blue: DAPI for nuclear staining.
