The GRK1 Antibody (CAB6497) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity specifically with rhodopsin kinase in human samples and has been validated for use in Western blot applications.Rhodopsin kinase is essential for the phosphorylation of rhodopsin, a light-sensitive protein in the retina that initiates the visual cascade. Dysregulation of rhodopsin kinase activity has been implicated in various retinal diseases, including retinitis pigmentosa and night blindness.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GRK1 Antibody
SKU:
CAB6497
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RK, RHOK, GPRK1, GRK1
Positive Sample:
Y79
Cellular Localization:
Lipid-Anchor, Membrane.
Calculated MW:
64kDa
Observed MW:
65kDa
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2).
Purification Method
Affinity purification
Gene ID
6011
RRID
AB_2619669
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Y79 cells, using GRK1 Rabbit pAb (CAB6497). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of paraffin-embedded rat retina using GRK1 Rabbit pAb (CAB6497) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
